Wednesday, January 26

 

 

 

14:00 – 22:00

Arrive: Hotel check-in and Meeting Registration

20:00 – 22:00

Light snacks & Social Gathering

 

 

 

 

Thursday (8:00 – 17:00), January 27

 

 

 

08:00

Poster setup (Odd-numbered posters)

 

 

 

08:30 – 10:10

Session 1:  T-Lymphocyte Subsets and Differentiation (Part I)

 

Chairman: M. Roederer (US)

 

R. Van Lier (NL):

Molecular basis of CD8+ T cell subset differentiation

 

M. Wills (UK):

Selection of the virus-specific CD8+ T cell repertoire following Primary Human Cytomegalovirus (HCMV) infection: TCR affinity and CD45RA+ CD28- memory cells.

 

D. van Baarle (NL):

Loss of CMV-specific CD4+ cytokine production and proliferative capacity during the course of HIV-infection is indicative for progression to CMV-End Organ Disease

 

 

 

10:10 – 10:30

Break (coffee and tea at the exhibits) and poster viewing

 

 

 

10:30 – 11:30

Session 1: T-Lymphocyte Subsets and Differentiation (Part II)

 

Chairman: M. Roederer (US)

 

S. Rowland-Jones (UK):

HIV-specific CTL: differentiation state and cytokine
secretion

 

A. Scheffold (D):

High-sensitivity immunofluorescence to detect specific peptide presentation at physiologically relevant quantities

 

 

 

14:00 – 16:00

BD Biosciences Tutorial:  Analysis of protein phosphorylation and cellular signaling events by flow cytometry.

 

 

 

16:00 – 17:00

Poster Viewing (coffee and tea at the exhibits)

 

 

 

 

Thursday (Evening), January 27

 

 

 

17:00 – 19:30

Session 2:  Cancer and New Technologies

 

Chairman: J. Gratama (NL)

       (Poster)

M. Streitz (D):

Systematic identification of T-cell epitopes in the human Cytomegalovirus proteome using spot-synthesized peptides

       (Poster)

C. Prussin (US):

Improved detection of allergen specific T cell cytokine responses using carboxyfluorescein succinimidyl ester enhanced T cell lines

 

A. Letsch (D):

T cell monitoring during vaccination against tumor-associated antigens

 

P. Lee (US):

Diversity and Recognition Efficiency of T Cell Responses to Cancer

 

P. Romero (CH):

Monitoring tumor antigen specific T-cell responses in cancer patients and clinical trials of peptide-based vaccination

       (Poster)

M. Frentsch (D):

Antigen-reactive CD154 expression facilitates analysis and isolation of the entire repertoire of antigen-specific CD4+ T-cells

       (Poster)

S. De Rosa (US):

Simultaneous examination of perforin, granzyme A and granzyme B in virus-specific T cells

 

 

 

20:00 – 22:00

Buffet Dinner, Exhibits, Poster Session I (Odd-numbered posters)

 

 

 

 

Friday (8:00 – 17:00), January 28

 

 

 

08:00

Poster setup (Even-numbered posters)

 

 

 

08:30 – 10:10

Session 3:  MHC & TcR Binding Technologies (Part I)

 

Chairman: F. Kern (D)

 

J. Altman (US):

Phenotypic analysis of tetramer binding cells

 

R. Volkmer-Engert (D):

Synthetic peptides and peptide mixtures for analysis of T-cell antigen recognition

 

D. Busch (D):

Ex vivo analysis of early events during CD8+ memory T cell development using MHC multimer technologies

 

 

 

10:10 – 10:30

Break (coffee and tea at the exhibits) and poster viewing

 

 

 

10:30 – 11:30

Session 3:  MHC & TcR Binding Technologies (Part II)

 

Chairman: F. Kern (D)

 

A. Thomsen (DK):

Profiling the optimal anti-viral CD8+ T cell response using MHC dextramers

 

T. Kuus-Reichel (US):

Immune monitoring using MHC tetramers: Potential clinical applications and practical considerations

 

 

 

14:00 – 15:00

Partec Tutorial: How the immune system starts its second century, presented by A. Cossarizza (Univ. of Modena); New perspectives for HIV monitoring: absolute volumetric counting of lymphocytes, CD45, CD4 and CD8 cells and quantitative viral load measurements by dedicated flow cytometry

 

 

 

15:00 – 16:00

DakoCytomation Tutorial: Tdex MHC Dextramers – Experiences and practical aspects in antigen specific T-cell analysis

 

 

 

16:00 – 17:00

Poster Viewing (coffee and tea at the exhibits)

 

 

 

 

Friday (Evening), January 28

 

 

 

17:00 – 18:00

Session 4:  B cells

 

Chairman: A. Lanzavecchia (CH)

 

N. Baumgarth (US):

Enumeration of B cell responses by flow cytometry

 

A. Lanzavecchia (CH):

Analysis of human B cell responses

 

 

 

18:00 – 19:30

Session 5:  In Vivo

 

Chairman: A. Lanzavecchia (CH)

       (Poster)

T. Bunde (D):

The CD8 T-cell response to the Immediate-Early-1 protein correlates with protection from CMV-disease in patients after heart or lung transplantation

 

R. Sekaly (CA):

Pathways of memory T cell development

       (Poster)

M. Betts (US):

HIV-infected long-term nonprogressors maintain polyfunctional HIV-specific CD8+ T cell responses of diverse memory phenotypes

 

R. Koup (US):

Comparison of antigen-specific responses between vaccines and natural infection

 

 

 

20:00 – 22:00

Buffet Dinner, Exhibits, Poster Session II (Even-numbered posters)

 

 

 

 

Saturday, January 29

 

 

 

08:30 – 10:10

Session 6: Infectious Diseases (Part I)

 

Chairman: F. Manca (IT)

 

G. Pantaleo (CH):

Antiviral memory T cell responses

 

B. Autran (F):

Antigen specific T cell responses and HIV pathogenesis

 

F. Kern (D):

Inroads into the phenotype and differentiation pathway of M. tuberculosis specific T cells

 

 

 

10:10 – 10:30

Break (coffee and tea at the exhibits) and poster viewing

 

 

 

10:30 – 11:30

Session 6: Infectious Diseases (Part II)

 

Chairman: F. Manca (IT)

 

G. Janossy (UK):

Technical developments to assess antigen-specific T cell responses in HIV and TB (co-) infections – the clinical need for discriminating rapid tests.

 

H. Maecker (US):

CMV and HIV-specific T cells in healthy individuals and HIV progressors

 

 

 

14:00 – 16:00

Beckman-Coulter Tutorial:  Novel technologies and approaches in epitope discovery

 

 

 

16:00 – 17:00

Coffee and tea at the exhibits

 

 

 

17:00 – 18:30

Session 7: Infectious Diseases (Part III)

 

Chairman: L. Picker (US)

       (Poster)

G. Li Pira (IT):

Cocktails of CD4 and CD8 peptides for measurement of CMV pp65 specific T cells and for generation of established Th and CTL lines

 

D. Douek (US):

T cell receptor recognition motifs govern immune escape by antigen mutation in acute SIV infection

       (Poster)

D. Price (US):

Dissection of clonal avidity relationships in antigen-specific CD8+ T cell populations directly ex vivo

 

L. Picker (US):

Analysis of T cell function in Simian Immunodeficiency Virus (SIV) infection: New approaches using polychromatic flow cytometry

 

 

 

20:30 – 22:30

Gala Dinner

 

 

 

 

Sunday, January 30

 

 

 

 

Depart